By adding the numbers corresponding to the positive reaction within each group, a7 digit profile number is obtained for 20 tests of the api 20e strip. Api stands for analytical profile index and is a commercial system to identify different bacteria. Oclcs webjunction has pulled together information and resources to assist library staff as they consider how to handle coronavirus. Api 20e analytical profile index system for identification. Api analytical profile index 20e presented is a biochemical panel for identification and differentiation of members of the family enterobacteriaceae. One of the api systems is specific for differentiating between members of the gram negative bacterial family enterobacteriaceae and is called api 20e. Jan, 2015 analytical profile index api api gram negative identification 1. The oxidase reaction constitutes the 21st test and has a value of 4 if it is positive. Sedangkan sistem api lainnya khusus untuk bakteri gram positif, termasuk. Result of api 20 e refers to the presence of escherichia coli 1 and escherichia 2 in the bacterial suspension. Api id strip range 10 api strips are used every minute in the world. Content of the kit kit for 25 tests 25 api 20 ne strips 25 incubation boxes 25 ampules of api aux medium 25 result sheets 1 package insert composition strip the composition of the api 20 ne strip is given in the reading table of this package insert.
Read book api 20e profile index manual api 20e profile index manual if you ally obsession such a referred api 20e profile index manual book that will have enough money you worth, get the unquestionably best seller from us currently from several preferred authors. According to the results provided by the api 20e kit, in six of the ten isolates of this microorganism in this study, the probability percentage id % was equal to or greater than 84. Salah satu sistem api khusus untuk membedakan antara anggota keluarga bakteri gram negatif dari family enterobacteriaceae dan disebut api 20e. Thenumberoftests makingupthertpis greaterthan thethreeorfourteststypically recommendedforeachstrain byapi. Identify your unknown organism in the api 20e analytical profile index book. It is hence a wellestablished method for manual microorganism identification to the species level. Analytical profile index which is also known as api is generally used for rapid identification of classified microorganisms. The api 20e test kit for the identification of enteric bacteria biomerieux, inc. Supplementary rapid biochemical test panelfor the api20e.
The tests in the api 20e kit and 52 conventional tests were separately evaluated for their ability to. Some of the wells will have color changes due to ph differences. Strains can store 2recently, the reliability of the analytical profile index api 20e identification procedure, commonly used to enumerate organisms in activated sludge systems, has come under. Oct 09, 2015 results and interpretations the 7digit profile is then compared with the numerical profile in the api 20 e analytical profile index book to obtain the organism identification. We compiled a simple panel of eight rapid 4h tests that can substitute for the supplementary. This api 20e kit contained 20 microtubes that contain dehydrated substrates. In api 20e for identification of members of the family enterobacteriaceae, the plastic strip holds twenty minitest chambers containing dehydrated media having chemicallydefined compositions. Analytical profile index or using the identification software. Api analytical profile index 20e presented is a biochemical panel for identification and differentiation of members of the family. Using results supplied by the manufacturer they found that in 9936% of 27 820 strains the identification shown in the api profile register was the same as that given by the program. Api20e system microbiology food and drug reference. Identification of enterobacteriaceae the api 20e system. Api abbreviation stands for analytical profile index.
The api and id 32 databases have again been updated twentytwo of the twentythree identification databases have been revised, taking account the biochemical profiles of over 56,277 strains. The 7 digit profile is then compared with the numerical profile in the api 20 e analytical profile index book to obtain the organism identification. Api analytical profile index 20e test procedure uses uitwerking experiment 8 food microbiology fhm 20306 studocu. Table 2 from identification of enterobacteriaceae by the api api 20e errors in identification id table api 20e identification and pathotec strip results asakawa y. The most advanced identification scheme so far developed for the api 20e kit the analytical profile index and complementary computer service allowed 88% of the 206 strains to be correctly identified, although 2% were incorrectly identified. The api 20e system is a miniaturized version of conventional tests that are used for the recognition of gramnegative bacteria.
Api identification products are test kits for identification of gram positive and. If the sevendigit profile that is tabulated cannot be found in the api 20e analytical profile index, what might that indicate about the bacterial culture. Apr 12, 2019 api analytical profile index 20e is a biochemical panel for identification and differentiation of members of the family enterobacteriaceae. Numerous and frequentlyupdated resource results are available from this search. Supplementary rapid biochemical test panel for the api 20e. Analytical profile index how is analytical profile index. If the sevendigit profile that is tabulated cannot be found in the api 20e analytical profile index, what. A total of 223 isolates belonging to motile aeromonas, a. A small amount was smeared over the filter paper and several drops of oxidase reagent were added and the color change was noted. Analytical profile index system for identificationof. A complete listing of comments built into the computer program can be found on pg 4 and 5 of the 20e analytical profile index. The api 20e system is a miniatuized version of conventional tsts that is used for the identification of members of the family enterobacteriaceae and other gramnegative bacteria. The api20e test kit for the identification of enteric bacteria biomerieux, inc. The most advanced identification scheme so far developed for the api 20e kit the analytical profile index and complementary computer service allowed 88.
Usefulness of the api20e system for the identification of. Api 20e identification system for enterobacteriaceae and other gram. What is the abbreviation for analytical profile index. This system utilizes a plastic strip with 20 separate compartments. This video was prepared by the teaching support team for the university of western australias school of pathology and laboratory medicine. To identify and differentiate members of family enterobacteriaceae. The method used for the api analytical profile index 20e system was the aseptic technique was used for the inoculating loop to get a unknown colony from the streak plate or pure culture slant. Ref 20 050 h 20 ne ivd florida international university.
When a profile number is not listed in the index, call the api computer service center 8006457056. Feb 23, 2014 result of api 20 e refers to the presence of escherichia coli 1 and escherichia 2 in the bacterial suspension. It was developed by analytab products, of plainview, new york. Analytical profile indexfor the problematic strains encountered in this study. Salah satu sistem api khusus untuk membedakan antara anggota keluarga bakteri gram negatif dari family enterobacteriaceae dan disebut api20e. The api 20ene fast identification system combines some conventional tests and allows. A plastic strip holding twenty minitest tubes is inoculated with a saline suspension of a pure culture as per manufacturers directions. The reproducibility of the analytab api 20e system for identification of enterobacteriaceae was evaluated with 110 clinical isolates. Api identification products are test kits for identification of gram positive and gram negative bacteria and yeast. Record results, obtain profile number and consult the api20e analytical profile index for the organisms identity. Api analytical profile index 20e test procedure, uses. Pdf reevaluation of the api 20e identification system.
Apiweb is rapid and easy to use key in the biochemical or numerical profile of. Each compartment consists of a depression, or cupule, and a. Api 20e analytical profile index pdf ebook biomerieux api 20e manual the analytical profile index or api is a classification of bacteria based on experiments, allowing fast identification. Api analytical profile index 20e test procedure uses. Discard the test system as you would a contaminated petri dish. Api 20e this api20e test strip f rom biomerieux, inc. Check out the new look and enjoy easier access to your favorite features.
Api analytical profile index 20e is a biochemical panel for identification and differentiation of members of the family enterobacteriaceae. Identifikasi bakteri dengan metode kit api 20e fakultas. The wellestablished method for manual microorganism identification to the species level, biomerieuxs api identification products are test kits for identification of gram positive and gram negative bacteria and yeast. Look up the numerical profile in the list of profiles.
Enterobacteriaceae and other gramnegative bacteria. Thesaline suspension wasalso inoculated onto plate count agar to test later for oxidase and catalase, and to ensure viability and purity. Identification of enterobacteriaceae by the api 20e semantic scholar. Results and interpretations the 7digit profile is then compared with the numerical profile in the api 20 e analytical profile index book to obtain the organism identification. The other api system is specific for gram positive bacteria, including staphylococcus species. Api 20e profile index api analytical profile index 20e is a biochemical panel for identification and differentiation of members of the family enterobacteriaceae. The api 20e system the api 20e system is a miniaturized version of conventional tests that are used for the identification of members of the family enterobacteriaceae and other gramnegative bacteria. If the sevendigit profile that is tabulated cannot.
Results and interpretations the 7digit profile is then compared with the numerical profile in the api 20 e analytical profile index book to. Catalogue analytique, analytical profile index, analytischer profil index. Api analytical profile index 20e test procedure, uses and. To identify gramnegative bacteria, regardless of oxidase reaction. Identification of enterobacteriaceae by the api 20e system. Learn api enterobacteriaceae with free interactive flashcards.
A plastic strip holding twenty minitest tubes is inoculated with a saline suspension of a pure culture as per. The principal advantage ofthe rtpis that reliable results are available in only 4 h, thus significantly reducingthe timerequired to completethe identifications. Refer to the instruction sheet by the phone for further details. The list of acronyms and abbreviations related to api analytical profile index.
Depending on the species, the api20e yielded false negative and positive reactions for fermentation of different sugars. A bacterial suspension is used to rehydrate each of the wells. The choice of microbiologists worldwide for its easeofuse and highperformance, the api id strip range is the global reference for identification. Analytical profile index api api gram negative identification 1. The api 20e analytical profile index typically suggests three or four conventional biochemical tests to complete the identification of strains either identified to genus only or that have multiple genera consistent with the profile number. The analytical profile index or api is a classification of bacteria based on experiments, allowing. Api 20e manual api analytical profile index 20e is a biochemical panel for identification and differentiation of members of the family enterobacteriaceae.